As promised, here is Project Marilyn’s 2015 budget. In it you can get a general idea how much it costs to run a science lab on a bare-bones budget.
One thing you will notice is that I broke out overhead into “scientific overhead” which specifically covers direct costs (such as renting lab space, hazardous material training and compliance, etc), which aren’t necessarily specific to Project Marilyn but without which Project Marilyn would not be possible. Of course, one of the major costs (labor) is not present because I’m operating Project Marilyn on a volunteer basis.
Not all of the purchases worked out. For example, the Liquid/Liquid extractor ($358) was a bit of a risk to try and improve the efficiency of 9DS extraction. But, on the other hand, buying it did make me realize that a soxhlet extractor ($108) was the correct apparatus to use. The lyophilizer (total ~$3000) was a big win, because the efficiency of 9DS production went up by somewhere around an order of magnitude due to that and the soxhlet extractor. Overall at the end of 2015, there was plenty of money still available for several months of continued lab work and the big purchase (xenograft mouse study).